Effects of Use of Combinations of Permeating Cryoprotectant (MeOH, DMSO) and Non Permeating Cryoprotectant (BSA) on Viability of Beluga (Huso huso) Post-Thawed Sperm

This experiment wascarried out to explore the effect of use of combinations of permeating cryoprotectant and non permeating cryoprotectant on thequality of frozen-thawed Beluga (Huso huso) spermas compared to the use of permeating cryoprotectant as a control. For this work methanol (MeOH) and dimethyl sulfoxide (DMSO)are used aspermeatingcryoprotectantsand bovine serum albuminas a non permeating cryoprotectant. Semen samples were pooled and divided into aliquots. MeOH and DMSO each at concentration of 10% was added to the extender; Tris-sucroseKCl (30mM Tris, 23.4mM sucrose, 0.25mM KCl, PH 8.0) and then semen was diluted with ratio 1:1 with extender and frozen in liquid nitrogen vapor. Onthe sametime BSA at concentration of 2.5,5 and 10 mg/ml and MeOH and DMSO at concentrations 10% was used that combined with the extender and then semen was diluted with ratio 1:1 with extender and frozen in liquid nitrogen vapor. Frozen sperms after 30 and 60 days were excluded from freezing. Experiment showed the highest motility duration and the most motility percentage of post thawed sperms after 30 days was related to the treatment combinatorial BSA at concentration of 10 mg/ml with DMSO10% (158.24±30.41 s and 20.41±2.18%; P<0.05), as well as the upmost mobility and the motility duration of post thawed sperms after 60 days was related to the treatments combinatorial BSA at concentration of 10 mg/ml with DMSO10% (135.58±20.47 s and 17.41±1.90%; P<0.05).